Insertion junction: CLIP2.058968_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058968
Chromosome:chromosome 6
Location:1724106
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g262750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGGGCTGCACCATGGGGCTGCTCCGGATTGCCAGTGTGCGTTGCGGG
Internal bar code:AACGGTCCTGTAGGGGATAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCGACCACTTTGGGTT
Suggested primer 2:CTGGTGGAGCAGGTCTTCTG