Insertion junction: CLIP2.058970_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.058970
Chromosome:chromosome 10
Location:5878897
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460650 CYN52 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 52 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGAGCTAACGAGCTTGAAATGGTCTTCGGTTGGACGGGCCTGGCCCA
Internal bar code:TGAGCTTATGTTCATAGTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1245
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCCCAGTCCAATGACCA
Suggested primer 2:CACCAGGGGCAAATCCTCAT