Insertion junction: CLIP2.058972_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.058972
Chromosome:chromosome 14
Location:444368
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g610700 PHC72 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGGCACTGAGCTCCAAGCGTCGCAATGGCACAGCGGGTGCACATGG
Internal bar code:GTACTAGCGGTGGTCTATTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1578
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCACAGCCTTCAACAC
Suggested primer 2:GCATCAAGGTGTGACCAGGA