| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058974 |
| Chromosome: | chromosome 2 |
| Location: | 4116070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103550 | EIF1A | Eukaryotic translation initiation factor 1A, eIF-1A; (1 of 1) K03236 - translation initiation factor 1A (EIF1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATAAGGTTCACCACATTGGTGGTGCCCATGTAACCCGCTCAGCATGC |
| Internal bar code: | GTGTAAGTCTTAGTCGCACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2100 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTCCGATACTGACCGTG |
| Suggested primer 2: | ACTAGGCAACCCTGAACACG |