| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.058984 |
| Chromosome: | chromosome 10 |
| Location: | 745036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTGCACGCAGGCAGGGTTTTGGCCAACCTCGATGATGGAGAAAGGTA |
| Internal bar code: | CTTTTATGTTAGAATTAAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCCTCCTTAAGAGCAA |
| Suggested primer 2: | TCCAAGAGCTCGTGTGACAC |