Insertion junction: CLIP2.058989_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.058989
Chromosome:chromosome 5
Location:272803
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g800512 (1 of 1) IPR011010//IPR013762//IPR023109 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain // Integrase/recombinase, N-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACAGTCCCTCTAGCGGGCTTCACGGCGGGCCGAGACGGGTTCAGAGCC
Internal bar code:TCCTAAGAAACTTCTGTCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1274
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTCTGTCTCCGATCGTG
Suggested primer 2:CGGGACCTGAGCGTGTATAC