| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059021 |
| Chromosome: | chromosome 12 |
| Location: | 7650244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555500 | (1 of 1) 2.4.2.21 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase / phosphoribosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTCTGTGATAAAGAGCACATGGGAGGAAGCACGCAAGACTGTGTGTA |
| Internal bar code: | TTAAGTGGTGGGGTCGGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1831 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCACATCGGAGCTAGG |
| Suggested primer 2: | TGACCGTTCACGAATACCCG |