| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.059029 |
| Chromosome: | chromosome 12 |
| Location: | 2169517 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGGAGCGAGACAGTGAGCAGTGCGTGTGCGGCTTATGTGGCTAGGG |
| Internal bar code: | GATAGAAGGAGTGTGGTCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACTCATGCGTGACGAA |
| Suggested primer 2: | TCCAGGAACAGGATCCGGAT |