| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059041 |
| Chromosome: | chromosome 10 |
| Location: | 92628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418150 | FAP178 | (1 of 1) IPR001715//IPR010441 - Calponin homology domain // CH-like domain in sperm protein; Flagellar Associated Protein 178 | 3'UTR |
| Cre10.g418200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGCGAATTGCCGAAGAGCGCAGACTCTTGCCAGCCTTTTAGCTTTCA |
| Internal bar code: | TTAGTAACTCGTGATTATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACCTAGACGAGAGGGG |
| Suggested primer 2: | TAGACGATGGTGGCGTATGC |