| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.059045 |
| Chromosome: | chromosome 10 |
| Location: | 4204320 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449200 | ANK5 | (1 of 1) PTHR22849//PTHR24133//PTHR24133:SF23 - WDSAM1 PROTEIN // FAMILY NOT NAMED // SUBFAMILY NOT NAMED; Predicted protein with ankyrin repeats | 5'UTR |
| Cre10.g449250 | KAP1,FLA3 | Kinesin II Associated Protein 1; (1 of 1) PTHR15605:SF2 - KINESIN-ASSOCIATED PROTEIN 3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTGAGAGGGCCGCAAATAGACTGTATTCTCATCACAGGCGCATTCT |
| Internal bar code: | GGAGATCACCCTCATCACACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1622 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACATGCAACGCCCTACT |
| Suggested primer 2: | TGTCCTCGCAATTCAGGCTT |