| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059050 |
| Chromosome: | chromosome 10 |
| Location: | 1935527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431800 | ARS5 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGTATTTTGAAAAGCTGACCTAGCTACAATCACCATAGCTAGTTGCA |
| Internal bar code: | CAGAATTTCATCGAAACTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4888 |
| LEAP-Seq percent confirming: | 98.4733 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCCGATGTATTTGTGC |
| Suggested primer 2: | TGCGTATACATTTGCAGCGC |