Insertion junction: CLIP2.059055_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059055
Chromosome:chromosome 4
Location:2941050
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224700 NRT1 (1 of 1) K14638 - solute carrier family 15 (peptide/histidine transporter), member 3/4 (SLC15A3_4, PHT); Nitrate transporter NRT1 family 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGGAGGAGGCGGAGGTGTGGAGGTGTGGAGGTGGGGTGGGGCCGAA
Internal bar code:TCAGTAATAACTCTGTCGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1991
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAACCCACACCTGGACTC
Suggested primer 2:GCGCTCTCAACTTTGCAACA