| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059060 |
| Chromosome: | chromosome 13 |
| Location: | 3304345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586000 | (1 of 1) K18660 - malonyl-CoA/methylmalonyl-CoA synthetase (ACSF3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTGCTTTGTTGGCCAAAAGGAATGCGGTCGGCAAACGCAAAGTGGG |
| Internal bar code: | GTGCCATCCTTGGTTTAACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTTTTGGCGAGCAACA |
| Suggested primer 2: | CATCCCTCCACACTCTGCAC |