| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.059065 |
| Chromosome: | chromosome 12 |
| Location: | 6090384 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535019 | (1 of 139) IPR029787 - Nucleotide cyclase | 3'UTR | |
| Cre12.g535050 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTCGGGGCACGCTGAGCCTGCAAGAATTGGATCACGCAGCAGGCTG |
| Internal bar code: | CTGTACAGGTTAAAGTACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCACGCACATAGCAGAA |
| Suggested primer 2: | GTTGGTACGGGGCGCATATA |