Insertion junction: CLIP2.059070_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059070
Chromosome:chromosome 17
Location:1286907
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g705300 PHC68 Putative pherophorin-chlamydomonas homolog; (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATCGACCACGGAGTGGATCGCCAAGGCGGCCTCATAAGTTGCGAGCCC
Internal bar code:TTAGTATTGACCACCTGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1452
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCAACATCCCCACCTCG
Suggested primer 2:GTATGGCATGTGGGTGGGAA