Insertion junction: CLIP2.059074_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059074
Chromosome:chromosome 8
Location:1523293
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365000 CSE1 Protein of unknown function; (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGCGTGATTCTTTCCGACTCTTCTGGTGCCCTTACCGTCTACGTGTG
Internal bar code:TAACTGCTAGGGGGTGCTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1357
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCATCATCACACATGCTG
Suggested primer 2:GTGAGGGCCTAGGTATTGCC