| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059081 |
| Chromosome: | chromosome 4 |
| Location: | 219595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217220 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAGGCTCAACGTTCGTGTAAGCCACCTGAGGTTCTAAAGTGACGAAG |
| Internal bar code: | AGCTGGAAATAAGCGGAGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 2.3622 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAATGGGACTCATGGGG |
| Suggested primer 2: | CAAACTTTGGTGGTCAGGCG |