Insertion junction: CLIP2.059112_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059112
Chromosome:chromosome 12
Location:7967343
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACGCCGCTCACCATGCGTATGTGCCCCGACATCGCGGGCATGGATGCG
Internal bar code:TTAAATGCGGAACACAGTGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGGACCCTATCGTGGAG
Suggested primer 2:CCCATCACGACACCATCACA