Insertion junction: CLIP2.059133_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.059133
Chromosome:chromosome 14
Location:2457085
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g623850 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAAATCGCGCATTTTGCCTGACGGCCAGGTGTGGCGCCCTGAGGAACT
Internal bar code:CACGGGTAAACGAGCGCGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:827
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCTGCACCTGTTCAGCA
Suggested primer 2:CCTGCTCCTCTGAAGCTGAC