| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059158 |
| Chromosome: | chromosome 7 |
| Location: | 2921439 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332650 | NCT1 | (1 of 2) PTHR11662:SF3 - INNER MEMBRANE TRANSPORT PROTEIN RHMT; High-affinity nicotinic acid transporter | 3'UTR |
| Cre07.g332700 | (1 of 1) PF00069//PF13639 - Protein kinase domain (Pkinase) // Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCGGTTCTGCCCTTTCTAACTCGGGTTTCTGGCTTTCTGGGTCAGT |
| Internal bar code: | CGAACGATACCGATTCTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCGTCATGTACACCACA |
| Suggested primer 2: | TTCTCCATGAACCGCTCACC |