| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059163 |
| Chromosome: | chromosome 14 |
| Location: | 3977263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633000 | ERV1,TOX1,ERV1A | Essential for Respiration and Viability 1; (1 of 2) K17783 - mitochondrial FAD-linked sulfhydryl oxidase [EC:1.8.3.2] (ERV1, GFER, ALR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACACGCGGGGTTGCGGTATTGCATATGCGGATGGCGAGGCCCAGGAG |
| Internal bar code: | ACATACGGGGGAGGATTTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2154 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTCACACAGCGCATTGA |
| Suggested primer 2: | AGTCCACACACACGCATCAT |