| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059183 |
| Chromosome: | chromosome 17 |
| Location: | 281688 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697850 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGTGCATCTGGAAGCTGATCCCCGTTCAGCGACGGGGTCTTCGGAGC |
| Internal bar code: | GTGTCACTGGTAATCTCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3895 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCGCACCATCATATCCC |
| Suggested primer 2: | AGTCACGCAGAGTTGTCAGG |