| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059184 |
| Chromosome: | chromosome 8 |
| Location: | 3811921 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTTCCTCGCGGTGCCCGGGAGGGATTCGCGCTAGAAGCTGCTAGCTC |
| Internal bar code: | ACTACTTTTTAAAATGTTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3181 |
| LEAP-Seq percent confirming: | 44.2308 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGAGCATAGCGAGTAGGC |
| Suggested primer 2: | GATAGATGGGGGCGGACAAG |