Insertion junction: CLIP2.059186_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.059186
Chromosome:chromosome 6
Location:4976586
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g281450 SRR22 Scavenger receptor cysteine rich (SRCR) protein; (1 of 1) 1.4.3.13//2.7.11.1 - Protein-lysine 6-oxidase / Lysyl oxidase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGGGTGCTGAGGCTGCGGACATGTGCGTGCGTGGAAGGTAGAGACGGC
Internal bar code:AAGGTTAATGGGATCACGCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1929
LEAP-Seq percent confirming:92.9577
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCAGGTGTGTGCATACGG
Suggested primer 2:CTTCTTCCCGCTTGTCCTGT