Insertion junction: CLIP2.059216_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.059216
Chromosome:chromosome 5
Location:873233
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246250 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type 3'UTR
Cre05.g246300 (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGAAAAGGCTTGAAGGCTTTCGCAAGTTGCGAGTCCCACGTGAGAGAC
Internal bar code:AGTGATACTATCGATAGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1200
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTGCGGTACAGTTTTT
Suggested primer 2:GACGTGCAATTGCTAGCCTG