| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059217 |
| Chromosome: | chromosome 12 |
| Location: | 8172850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552006 | 3'UTR | ||
| lncRNA_TCONS_00121246 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGCTTGCCTGGGCCGTGCGGCCGTGCGGGATTCACAGCGCCGCCGC |
| Internal bar code: | CTGGCGTGACTGTCCTAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1571 |
| LEAP-Seq percent confirming: | 59.2105 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGACAGGGAGAGGGGTGA |
| Suggested primer 2: | TGCCGTGGCTTGATAACCTT |