| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.059226 |
| Chromosome: | chromosome 17 |
| Location: | 1931890 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709950 | (1 of 1) K08272 - calcium binding protein 39 (CAB39, MO25) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGATGACTAGAGCGTGCGGTGGAAATGCCCAAGCGGAAGAGCTGAGC |
| Internal bar code: | CCTCGTTTTGAACTAGTGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2573 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTAGGTCTTAAGCCCG |
| Suggested primer 2: | TAGCCGTTCATGCAGCGTAT |