| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059230 |
| Chromosome: | chromosome 7 |
| Location: | 6061328 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355200 | ORC5 | Origin recognition complex subunit 5; (1 of 1) PTHR12705//PTHR12705:SF0 - ORIGIN RECOGNITION COMPLEX SUBUNIT 5 // ORIGIN RECOGNITION COMPLEX SUBUNIT 5 | 3'UTR |
| Cre07.g355250 | WEE1 | CDK inhibitory kinase; (1 of 1) K06632 - wee1-like protein kinase (WEE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCTTGATTGTTTGCCGGGACGGAATATGGTATGGTTCGCCAAGATG |
| Internal bar code: | GACGCAGGGTCTCCAGGTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACAGGCTGCCGTTAGAG |
| Suggested primer 2: | ACCTTATCCTGACCTCCCCC |