Insertion junction: CLIP2.059234_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059234
Chromosome:chromosome 3
Location:554596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145247 TEF11 S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAACACACATACTTAGTTTCGAGACGTCTGAAATTTTCTATACTATT
Internal bar code:GTGGATCGTGAAGTAGTGCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2120
LEAP-Seq percent confirming:73.6842
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACTGGGCTCAAAAGACG
Suggested primer 2:TGGGCGCTATGTGGATTGTT