| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059234 |
| Chromosome: | chromosome 3 |
| Location: | 554600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145247 | TEF11 | S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCTGCGGACCCGGATTCGCGAGCTGGCTTCGGACCTGGTTTCGGGG |
| Internal bar code: | ACTTACAGCCTGAGACTGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1800 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGCGCTATGTGGATTGTT |
| Suggested primer 2: | AGGACTGGGCTCAAAAGACG |