| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059235 |
| Chromosome: | chromosome 2 |
| Location: | 6989651 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389467 | OPR8 | (1 of 7) IPR014721 - Ribosomal protein S5 domain 2-type fold, subgroup; OctotricoPeptide Repeat protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACAGACAGGCGTTTTGGCACACGCTGAAGCACGACGGTCCGAAGGCC |
| Internal bar code: | CGGCGAGCGTGGGCGTGTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1531 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCACGCTTTGTTTGTG |
| Suggested primer 2: | CACACACACACAAGCATGCA |