| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059253 |
| Chromosome: | chromosome 8 |
| Location: | 4062707 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGTGTGGGTGTTGGA |
| Internal bar code: | CAGTGCGCCATAGATTCATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 53.6585 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACGTCAAAGCCCACAC |
| Suggested primer 2: | CACTTGGCTTCAAAGTCCGC |