| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059254 |
| Chromosome: | chromosome 4 |
| Location: | 4072515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233202 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | 3'UTR | |
| Cre04.g233302 | (1 of 2) PTHR23132//PTHR23132:SF9 - D-ALANINE--D-ALANINE LIGASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATACTATACGAGGACATGCAGGAGCGGCTGGTCTGGCGGTTTAAT |
| Internal bar code: | TTAATAAATATCCTACCTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2467 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTCAGTGTGCTCAAGCT |
| Suggested primer 2: | CAATCTTTACTCGTGCCGCG |