Insertion junction: CLIP2.059258_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059258
Chromosome:chromosome 2
Location:2796237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094250 (1 of 2) K13354 - solute carrier family 25 (peroxisomal adenine nucleotide transporter), member 17 (SLC25A17, PMP34) 5'UTR
Cre02.g094300 FUO1 (1 of 1) 1.5.5.1 - Electron-transferring-flavoprotein dehydrogenase / ETF-ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGCAATACGCCTGGTGCGGGGGTTGAACCCGCAGTGTCAAGGGGAAAT
Internal bar code:TCTTATGCACATGATAAATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2680
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGATTCGTCGGGAACTCG
Suggested primer 2:TCAAGGACGAAGGCGAGTTC