| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059259 |
| Chromosome: | chromosome 16 |
| Location: | 7954138 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690991 | weak Similarity to Elongation factor 1 | gamma chain; (1 of 1) K03233 - elongation factor 1-gamma (EEF1G) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCACATACCATACATGCACTGCCCCTGCACCTTTCAAGACTGGGCAA |
| Internal bar code: | ATGTGCGCGGAGGGAAGCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2252 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCATCTCTCGTGGAGCG |
| Suggested primer 2: | CCTGCTGTGTCAAAGGTTGC |