| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059275 |
| Chromosome: | chromosome 2 |
| Location: | 8476431 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142000 | CrUbcE2_1,CrUbc9,CreSCE1a,CrSCE1,UBC4,SCE1 | (1 of 1) PTHR24067//PTHR24067:SF153 - UBIQUITIN-CONJUGATING ENZYME E2 // SUBFAMILY NOT NAMED; SUMO-conjugating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCCTGCTCAGTGCTGCAAGAGCAAGAATGCACACACCACGGCCCGTC |
| Internal bar code: | GACCTGTTGCATCCGGGACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGACGGACGTGCAGGAG |
| Suggested primer 2: | CTCATGGCCATATGCGGGAT |