Insertion junction: CLIP2.059279_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059279
Chromosome:chromosome 16
Location:2675762
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGGTTGTGCGGCACAGGTGACTGACGCGCCAAGCAGTCGACGTCTCG
Internal bar code:GAGTAGTTTCCTGTAAGAGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3126
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GACCCACCTAGCCTGCTTTT
Suggested primer 2:GACAGCGCTTCGAGACGATA