Insertion junction: CLIP2.059289_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059289
Chromosome:chromosome 12
Location:7240767
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g558900 PETO1,PETO Regulator of photosynthetic cyclic electron flow 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGTTCGGCTGTCCGCGAGGTTGCACGACCTCGAAGCTGCATGAGGAGC
Internal bar code:GTCTAGGCCAATGTTTGACCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4045
LEAP-Seq percent confirming:98.7013
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCGAGCAGTTTGTTGGCG
Suggested primer 2:TTTGGGTAGCGGCAACTAGG