| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059291 |
| Chromosome: | chromosome 6 |
| Location: | 2135111 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800632 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTCGTGATTTACGCAGCCCGGTCAGTGTTGCGTTTTTTTAAGCACGT |
| Internal bar code: | TTCACGTTGACCGCAATCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2821 |
| LEAP-Seq percent confirming: | 0.925926 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGATCGAGGGACGTAGGC |
| Suggested primer 2: | CGTACACAGGCCTCGGATAC |