| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059308 |
| Chromosome: | chromosome 9 |
| Location: | 4590213 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401478 | (1 of 1) PF03232 - Ubiquinone biosynthesis protein COQ7 (COQ7) | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATGACCCCCGCGCTCCCTCGCCTCCGGAGTAGAGGGGAGGAACCCCT |
| Internal bar code: | ATTAATCCGCCGGGTTTTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6235 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTTCACGCGTGCAAAGA |
| Suggested primer 2: | GAAGCGGTGTGGTTTTGTCC |