| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059308 |
| Chromosome: | chromosome 9 |
| Location: | 4590215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401478 | (1 of 1) PF03232 - Ubiquinone biosynthesis protein COQ7 (COQ7) | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGTCTTCTCGTACGTGTCTAGTACGTTTTTGGGGCCCTGGCTTAGTC |
| Internal bar code: | CGCTGATACTGGTACGAAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCGGTGTGGTTTTGTCC |
| Suggested primer 2: | CAGTTTCACGCGTGCAAAGA |