| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059318 |
| Chromosome: | chromosome 10 |
| Location: | 3933350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446750 | (1 of 2) PTHR13690//PTHR13690:SF71 - FAMILY NOT NAMED // PROTEIN C37C3.9 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGACAACATCGTGCCTCCTGGTGCCTCCCGAATTCGATGCGCTTGGG |
| Internal bar code: | GCGACTACCTTAGCTTCCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4377 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATTTTGGGCTTGCTGAA |
| Suggested primer 2: | GCGACGATTGAATTCCACGG |