| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059330 |
| Chromosome: | chromosome 8 |
| Location: | 285867 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358568 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAGGCGGGGGCTGGCCGACTGCAAACAATACTTCGCGAGGAAGTTCG |
| Internal bar code: | GGTCTAGCCTCACAGTCGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1205 |
| LEAP-Seq percent confirming: | 95.8904 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTTGAACAGCGTTTCC |
| Suggested primer 2: | CGCCTTGGGAACACACTTTG |