| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059332 |
| Chromosome: | chromosome 13 |
| Location: | 1598667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573300 | CDD4 | (1 of 1) K11991 - tRNA(adenine34) deaminase [EC:3.5.4.33] (tadA); Cytosine deaminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTAATAATAGGGCGGAATCTGGAGAAATGTCCTGAACTTGACATACC |
| Internal bar code: | ATCGTAGTCCGGCCGTGTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3890 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTTGCGTGTTCAACCCG |
| Suggested primer 2: | GCCAACAGCACTGCATGATC |