| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059339 |
| Chromosome: | chromosome 16 |
| Location: | 4946549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686550 | (1 of 1) PF12400 - Vaculolar membrane protein (DUF3661) | 3'UTR | |
| Cre16.g686552 | (1 of 1) 3.6.1.12 - dCTP diphosphatase / Deoxycytidine-triphosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGCAGCCCGCTCTTGCCAAGTGGCACCTGATGCGTACATACGGTAT |
| Internal bar code: | AAGGTTAGATAGCCTGATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGGAACTTGAGGAAGG |
| Suggested primer 2: | CATGCCGGCATGTGGTATTG |