Insertion junction: CLIP2.059353_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.059353
Chromosome:chromosome 1
Location:5452494
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g038550 SQD2 Sulfoquinovosyldiacylglycerol synthase; (1 of 2) K06119 - sulfoquinovosyltransferase (SQD2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTAGGGTTTGCGCCAGAGAACAAGTGAGCCATATGTGATTTTGTGCGTG
Internal bar code:AGGTAGGAGAATAAAGTGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2099
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGGGTGGAGCAGTCTGAG
Suggested primer 2:CGCAGATGCCTGAGGGTAAA