| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.059354 |
| Chromosome: | chromosome 11 |
| Location: | 2156092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467739 | IFT54,Traf3ip1,FAP116,Dyf-11Elipsa | Intraflagellar Transport Protein 54; (1 of 1) PTHR31363:SF0 - TRAF3-INTERACTING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGATGGCGCTTTAGCTTGTCCTGCTCGATCTTGCGGATGAGGCTGG |
| Internal bar code: | CAGGACGGGTCGGCACAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1954 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACAGTGTTTGTCGTGCC |
| Suggested primer 2: | TGTGTTACTGGGATGTGGCC |