| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059357 |
| Chromosome: | chromosome 5 |
| Location: | 1144203 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248401 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCAGACCCGGCCACCAGATGCGTCGCTCCGGGTCGTACACGCAGTGT |
| Internal bar code: | GGTTGTGTCGAAAAATTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2420 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCGTCTGTTGACAGCTC |
| Suggested primer 2: | ACAGCTTACAGGGCATGCAT |