| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059367 |
| Chromosome: | chromosome 17 |
| Location: | 365004 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698450 | (1 of 3) 3.5.4.9 - Methenyltetrahydrofolate cyclohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCGCCCGAGCATTCCCCCCAGGAAATCTGTCTCATCCAGAATCTTC |
| Internal bar code: | CAAAATGGTTTGGCAAAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAAAGCAGTGGGAACAG |
| Suggested primer 2: | TTGTAAAGCCACTTGTGCGC |