| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059380 |
| Chromosome: | chromosome 12 |
| Location: | 6297060 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536650 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGCGACGACCCGCACCCAGCTCACCTATGCTCGCCTCGCTATCTCC |
| Internal bar code: | AGTATCTTGCGATTTTGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2342 |
| LEAP-Seq percent confirming: | 35.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGTGCAGAGGAACAAGA |
| Suggested primer 2: | CTCATTGTTGCGCTCGTAGC |